Cells were passaged at confluence with 0.05% trypsin and maintained in a 37 C incubator with humidified atmosphere of 5% CO 2 and 95% air
It has also been shown to influence lipid metabolism enzyme activity, including hormone-sensitive lipase and lipoprotein lipase, and to modulate adipogenic transcription factor expression in adipocyte differentiation models
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These entities included government bodies, public and international financing organizations, and donors, as well as international implementing entities: National Agency of Water Supply, Sewerage, and Waste Infrastructure (AKUM), the Swedish Embassy SIDA, Ministry of Infrastructure and Energy, the German Development Bank (KfW), European Union Delegation, World Bank in Albania, the Swiss Embassy, Water Supply Sector Regulator Authority (ERRU), the Agency for the Management of Water Resources (AMBU), the Ministry of Health and Social Protection, the Ministry of Agriculture and Rural Development, the Ministry of Finance and Economy, the National Institute of Public Health (NIPS), The National Environment Agency (AKM), the Albanian Association of Water Supply and Sewerage (SHUKALB), Austrian Development Agency, SECO , SANE27 and SETEC-SWECO team.

Binds extracellular and exchangeable copper(II) ions with high affinity, facilitating copper delivery into cells and enhancing the action of copper-dependent enzymes necessary for extracellular matrix synthesis and antioxidant defenses 2