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Following collection, adipose tissue explants were rinsed in phosphate-buffered saline (PBS) (P4474, Sigma-Aldrich, Pozna, Poland) supplemented with 1% penicillin/streptomycin (P/S) (P4333, Sigma- Aldrich, Pozna, Poland) to prevent microbial contamination and then transferred into transport medium consisting of Dulbeccos Modified Eagles Mediumlow glucose (DMEM-LG containing 1000 mg/L of glucose) (D9443, Sigma- Aldrich, Pozna, Poland) supplemented with 1% P/S

First-visit patients also sign a basic consent

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