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3b, upon reduction with 2-mercaptoethanol, glutathione was released between SEC fractions 37 and 41

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Biotin-enhanced formulas Some ALA supplements include added biotin as a precaution, based on limited evidence primarily from animal studies suggesting high doses of ALA might interfere with biotin metabolism.57 While the real-world significance in humans remains unclear, manufacturers include biotin to help prevent any potential deficiency

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