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The GHSRGiscFV16 complex was purified and buffer-exchanged by size exclusion chromatography with buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 0.002% MNG, 0.001% CHS, and 1 M ghrelin or ibutamoren

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For those who are visual learners, our team and collaborators often break down complex topics and procedures on channels like the MorelliFit YouTube channel, which can be a fantastic supplementary resource