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Isocratic enantiomer separation was at a flow rate of 1 mL min 1 at 27C using a mobile phase consisting of 85% (v/v) 100 mM ammonium acetate, 1 mM Cu(II)-acetate (pH 4.5, with acetic acid) and 15% (v/v) 2-propanol

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Cell cycle was analysed on a BD LSR II flow cytometer (BD Biosciences) and data were analysed using ModFit software

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Microglial replacement using Ki20227, a CSF1R inhibitor, in 2HIT mice restored behaviors resembling psychiatric disorders, suggesting a causal contribution of 2HIT stress-reactive microglia, including SAM (Hikosaka et al., 2025)