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The relative copy numbers of mitochondrial to nuclear DNA were determined by real-time PCR with primers specific to cytochrome c oxidase subunit II (COX II) (mitochondrial) 5-ATCCCAGGCCGACTAAATCA (forward) and 5-TTTCAGAGCATTGGCCATAGAA (reverse) and -actin (nuclear) genes 5-TATCCACCTTCCAGCAGATGT (forward) and 5-AGCTCAGTAACAGTCCGCCTA (reverse)

Bie P, Wamberg S, Kjolby M

Benign vocal cord lesions are noncancerous growths that may form on one or both vocal cords

Ethics statement The animal study was approved by Agricultural University of Hunans biomedical research ethics committee (No: 2023096)