INTRODUCTION Methotrexate (MTX) is a synthetic antimetabolite with many therapeutic indications, mainly anti-inflammatory and immunosuppressant at relatively low doses and antineoplastic at high doses.[ , ] MTX competitively inhibits dihydrofolate reductase, so decreasing tetrahydrofolate level leads to blocks the synthesis of purines, pyrimidines, DNA, ribonucleic acid, thymidylate, and proteins.[ , , ] It is well known that MTX is a hepatotoxic drug, and this leads to limitation of its clinical use
In this study we demonstrate that the fusion of human glutaredoxin-1 (Grx1) to roGFP2 facilitates specific real-time equilibration between the sensor protein and the glutathione redox couple
Induction of glutathione S-transferase genes of Nicotiana benthamiana following infection by Colletotrichum destructivum and C
FEBS Lett 580:11031111 Dhanani S, Awasthi YC (2007) Glutathione S-transferase isoenzyme composition of human tissues
Quantitative prediction of human pharmacokinetic responses to drugs via fluidically coupled vascularized organ chips
