Pay in 4 interest-free payments of $7.21 Learn more
Ships within 48 hours · Estimated delivery Aug 7 - Aug 12
B.Dos Santos CabreraM
Its the sweet spot of effectiveness without overwhelming the bodys natural processes
10.1210/en.2013-1934 33 RajanS.DicksonL
FACS trafficking MIN6B1-SNAP-GLP-1R or CHO-SNAP-GLP-1R cells were pre-incubated in HEPES-bicarbonate buffer plus 3 mM glucose and 1% BSA before labeling at 37 C with 1 M cleavable BG-SS-488 SNAP-Surface probe (a gift from New England Biolabs) and stimulation with 100 nM agonist in HEPES-bicarbonate buffer plus 11 mM glucose and 1% BSA, and trafficking assays performed as follows 27 : Briefly, for receptor internalization, cells were placed at 4 C to arrest endocytosis following incubation at 37 C for the indicated times, or directly put at 4 C for 0 min time-point, and treated with ice-cold alkaline TNE buffer 100 mM MesNa to strip surface-exposed label (plus MesNa) or to measure total labeled receptor (minus MesNa)
K.ChengY.KanwarM
US$ 21.35
US$ 23.54
US$ 28.31
