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Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 l ice-cold PBS and fixed by adding 700 l ice-cold absolute ethanol while gently vortexing

Endocrinology 142 , 19231934 (2001)

The CDC's safe injection practices guidelines state that "single-dose vials and syringes should never be used for more than one patient" and that "needles should be discarded in a sharps container immediately after use" [11]

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-- Personal note from Jordan I have always been fascinated by how our bodies try to protect us from ourselves