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To verify these conditions optimized for peptide 1 , the longer peptide sequences 18A ( 2 ), an amphipathic helical peptide 19 , and GHRH(1-29) ( 3 ), the active component of growth hormone-releasing hormone 20 were then synthesized in full automation and analyzed by reversed-phase HPLC (RP-HPLC) and electrospray ionization mass spectrometry (ESI-MS) (Fig