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The filtrate was added to 40 L of ice-cold anhydrous diethyl ether, precipitated, centrifuged, washed with anhydrous diethyl ether, and dried under vacuum to obtain 182 g of crude Semaglutide with a purity of 66.8%

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In the Netherlands, stepwise treatment for T2D begins with biguanides such as metformin, followed by the addition of a sulphonylurea when biguanides do not adequately control hyperglycaemia, followed by the addition of basal insulin and eventually intensive pharmacotherapy with insulin [9]