As for the increase in the alpha-helical content with substitution of value with proline, we note that despite proline being accepted as the helical breaker in an aqueous medium, reports of its function otherwise have been observed when they are in membrane environments than in water
Huang HY, Helzlsouer KJ, Appel LJ
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Microscopy Brightfield and fluorescence microscopy were carried out on an Olympus IX70 inverted microscope
Ethics statement The animal study was approved by Ethics Committee of Laboratory Animal Welfare, Affiliated Hospital of Qingdao University