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Mouse anti-eNOS and anti-phospho-Ser-1177-eNOS (p-eNOS) antibodies were from BD Biosciences (San Joe, CA, USA)

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This oxidative stress triggers various signaling pathways, including mitochondrial and endoplasmic reticulum pathways, leading to the release of Cyt-C and apoptosis-inducing factors, ultimately resulting in nerve cell apoptosis (Wang and Huang, 2012