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This ensures the B12 remains in the muscle for steady release rather than being dispersed too quickly

While I have never used expired b12 vials since a pharmacist told me not to I have used multi-use vials after 28 days when they are supposed to be discarded and unrefrigerated once opened as well

PMID: 30281517 7 Primary and maternal 3-methylcrotonyl-CoA carboxylase deficiency: insights from the Israel newborn screening program

Research subjects typically administer 0.1 to 0.3 mg via subcutaneous injection 30 to 60 minutes before bedtime, with cycles running 4 to 8 weeks followed by a break period

PMID: 27306034 View source Sikiric P, et al