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Understanding how it compares to alternatives helps optimize protocol selection

neonatal biochemical screening for disease

Although no formal a priori power calculation was performed, the sample size of six animals per group (total n = 24) was selected based on commonly used group sizes in comparable rodent models of skeletal muscle I/R injury, in which cohorts of six to eight animals are frequently employed for the combined evaluation of biochemical, molecular, histopathological, and immunohistochemical endpoints

PMID 6207965

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