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To account for the extent of multiple inclusion of primary studies (overlaps) in the included systematic reviews, the Corrected Covered Area (CCA) formula was used: where N is the sum of the included primary studies, accounting for double counting, r is the number of distinctly indexed primary studies, and c is the number of included systematic reviews ( 2.5 Quality assessment AMSTAR-2 guidelines were used to assess the methodological quality of the included systematic reviews and meta-analyses

In another embodiment, the spacers include both regions that are neutral and regions that may be protonated to carry one or more positive charges

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( Erratum in: Eur J Nutr