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After tipping off the supernatant, plates were washed, blotted on paper, and blocked with PBS 1% BSA for 30 min at RT

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Knockdown of gss- 1 has also been shown to increase the fluorescent intensity of the gcs-1 ::GFP reporter, indicating a regulatory control mechanism exists to increase gene expression for the first step of the GSH biosynthesis pathway when the second step becomes compromised [81]