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C2C12-IR IGF1R knockout C2C12 cells expressing human IR-A( 41 ) (C2C12-IR) were cultured in high-glucose DMEM supplemented with 10% (v/v) FBS, 2 mM L-glutamine, and 1% penicillin/streptomycin and maintained in monolayer culture at 37 C and 5% CO2 incubator

Together, these findings suggest that VRK2-deficient PC cells exhibit a metabolic preference for GSH dependency, implying that VRK2 has the potential to regulate GSH biosynthesis

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