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Schecklmann M, Landgrebe M, Poeppl TB, et al

A1AT contains methionine residues that are highly susceptible to oxidation by ROS, particularly singlet oxygen and hydroxyl radicals, resulting in the formation of methionine sulfoxide

We then performed luciferase reporter analyses by transfecting HEK293T cells with these constructs, either alone or in combination with P65, or by treating the cells with TNF

In a different aliquot, the same extract was initially added with 200 mM phosphate buffer (pH 7.4) and 200 L 10 mM dithiothreitol (DTT) to reduce all dehydroascorbate (DHA) into AsA and continued the same reaction as mentioned

Structural datasets of this kind are typically deposited in public repositories, enabling independent reanalysis