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To gain insight into the role of the basic cluster in stabilizing S-coatomer interactions, we generated alanine mutants of WD40 Arg13, Lys15, and Arg300, which are equivalent to the WD40 basic cluster residues Arg15, Lys17, and Arg272, respectively

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anti-GCLC (rabbit, 1:1000, Bioworld Technologies)

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