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Analysis of tissue for antioxidant activities Previously frozen testis (20% w/v), kidney (20% w/v), and liver (20% w/v) tissues were swiftly thawed and homogenised with PRO 200 homogeniser (Proscientific Inc, Oxford, USA), in 1 ml ice-cold Tris-buffered saline (TrisHCl 20 mM, NaCl 150 mM, pH 7.4) and centrifuged (5,000 g , 30 min and 4 C)