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Quality criteria include blue-colored lyophilized powder (confirming copper coordination), 99%+ HPLC-MS verified purity, and per-lot Certificates of Analysis

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After filtration and centrifugation, the cell pellet was resuspended with low glucose Dulbeccos modified Eagles medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS, Gibco) and 1% penicillinstreptomycin (Gibco) at 37 C and 5% CO2 in 10-cm dishes

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