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For example, curcumin in nanoformulation and liposomal encapsulated has been investigated to circumvent poor systemic absorption and enhance targeting into tumour tissue to permit accurate discovery of dose-response relationship as well as pharmacodynamic effects in vivo (229)

However, this prolongs and complicates the separation process ( In contrast to animal EXOs, plant EXOs have a wider size range (50500 nm) (Kumar et al., 2023b), which makes it challenging to extract a homogeneous population utilizing size-based separation methods like size exclusion chromatography or differential centrifugation (Tian et al., 2023)

These fractions were pooled and used for activity and immunological analysis as shown in Figures 4 and 5