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Outcome measurements

Preliminary experiments The purposes of the experiments were to determine the optimum pH of the buffer solution for non enzymatic and enzymatic reactions of monobromobimane (MBB) with glutathione (GSH) as well as the quenching effect of the presence of excess MBB in a typical reaction mixture on the fluorescence of the reaction product

The Poisson correction method (Zuckerkandl and Pauling, 1965) was employed to calculate evolutionary distances, expressed in units of the number of amino acid substitutions per site

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A glutathione injection delivers glutathione directly into the body, where it supports antioxidant activity, helps manage oxidative stress, and plays a role in normal cellular processes related to detoxification and overall wellness