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Measurement of average telomere length DNA was extracted from ovaries using TIAN amp Genomic DNA Kit (Tian Gen Biotech CO., Ltd., Beijing, China) as described by the manufacturer and absorbance at 260 nm measured with a spectrophotometric plate reader (BioTek, Winooski, Vermont, USA)

Selective NNMT inhibitors (including 5-Amino-1MQ) lower 1-MNA, increase NAD and SAM, suppress adipocyte lipogenesis, and reverse HFD-induced obesity in mice [1]

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It can therefore contribute to impaired methylation, but high SAH should not automatically be labeled toxicity without broader evaluation