Using 50 Ethicon Mersilk suture (Ethicon, Norderstedt, Germany), 50% of the cecum was ligated and subsequently perforated by a single through-and-through puncture with a 22 G needle (for mild CLP), or 100% of the cecum was ligated and subsequently perforated twice by a through-and-through puncture with a 20 G needle (for severe CLP)
Studies show that the C667T and A1298C variants increase the relative risk : high homocysteine, stroke, and heart disease[ref][ref][ref][ref][ref] neural tube defects and cleft lip[ref] preeclampsia and hypertension in pregnancy[ref][ref] miscarriage[ref][ref] depression or anxiety[ref][ref][ref][ref][ref] Alzheimers[ref][ref] autism spectrum disorder (C677T only according to 2026 meta-analysis)[ref] rare problems with nitrous oxide[ref], but most have no problems[ref] Lets dig into the details of what research studies and clinical trials show
Autophagy and mitophagy promotion in a rat model of endometriosis
AO, FR, CF, and LB: supervision and funding acquisition
The focus is chosen not only because of the plethora of research on these conditions, both of which are highly prevalent in 22q11DS, but also because of the increasing number of other rare pathogenic genetic variants identified, which appear to have similarly increased rates of ASD, SSD, or both (e.g., 16p11.2 deletion, 3q29 deletion, 1q21.1 deletion)
