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2.10 Chromatin immunoprecipitation assay Cardiomyocytes were crosslinked with 1% formaldehyde(Sigma-Aldrich, Cat# F8775) for 10 minutes at 37C, and a ChIP assay was performed using EZ-Magna ChIP A/G Chromatin Immunoprecipitation Kit(Merck Millipore, Cat# 17-10086) according to the manufacturers instructions

Effect of metformin on stem cells: molecular mechanism and clinical prospect