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The hepatic thawing and seeding medium was replaced by hepatic perfusion medium composed of Williams Medium E, 10% (v/v) FCS, 3.6% hepatocyte maintenance supplements CM4000 (GIBCO), 0.1 M dexamethasone, 2 mM L-glutamine, 5 g/mL insulin, 5 M hydrocortisone-hemisuccinate, 0.1% DMSO and AAS after 24 h