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The samples were centrifuged at 1000 rpm for 3 min using a KUBOTA 3520 (KUBOTA, Tokyo, Japan), and absorbance was measured at 595 nm using a GeneQuant 1300 spectrophotometer (Biochrome, Cambridge, UK)

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By simultaneously activating the GLP-1, GIP, and Glucagon receptors, this compound is currently the subject of intensive study regarding its potential impact on metabolic efficiency and energy regulation