Venography assessment includes rats having a full presentation of collaterals (i.e., presentation of pterigopalatinal veins and nasal veins and presentation of the ophthalmic vein, angularis vein, facial anterior and posterior vein, and facial vein, or through cerebri superior veins, sinus cavernosus, sinus petrosus superior and inferior, sinus transversus, through jugular external vein, subclavia vein through superior caval veins after venography into the superior sagittal sinus
Diabetologia 56 , 13941402 (2013)
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ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well
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