Additionally, the TME underwent significant remodeling, resulting in a 14.8-fold enhancement in T-cell survival and proliferation in vivo and a 5.4-fold increase in vitro
GLP-1 receptor agonists are FDA-approved for two primary indications: type 2 diabetes management and chronic weight management in adults with obesity or overweight with at least one weight-related comorbidity
2.6 Analysis of oxidative stress SOD activity, glutathione (GSH) content, and malondialdehyde (MDA) levels (all of which are indicators of the oxidative stress burden) were measured in hepatic tissue homogenates using the colorimetric kits obtained from BioVision, Milpitas, CA, United States: SOD kit (Cat: K335100), GSH kit (Cat: K464100), and MDA kit (Cat: K739100), according to the guidelines provided by the manufacturer
ab179832, Abcam) at 4 C, overnight
Sections were washed in PBS three times for 5 min each and were incubated with secondary antibody for 45 min