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Screen-based detection of intracellular cAMP Islets were plated in clear-bottom 96-well plates with KRB containing 2.8 mmol/l glucose and stimulated for 20 min at 37C with KRB (20 mmol/l glucose) plus IBMX (500 mol/l), either alone or combined with forskolin (10 mol/l), GLP-1 (1 nmol/l) or triagonist (1 nmol/l)

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Supplementation becomes more important when glutamine stores are depletedespecially after hard training sessions, fasting, or illness

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