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Here we used a combination of both LO-NMR and X-ray crystallographic fragment screening to identify a first-in-class series of fragment hits that bind to TRF1 TRFH and inhibit the interaction between TRF1 TRFH and TIN2 TBM with medium-micromolar potency as measured by a fluorescence polarisation (FP) competition assay

The new findings for ImmH in the context of NAD + synthesis potentially provide another strategy for indirect NAD + upregulation that requires further investigation

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Laboratory studies demonstrate that while the compound remains stable at room temperature for weeks, refrigeration significantly minimizes degradation and extends usable research timeframes