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The metformin dosage was calibrated and adjusted according to body surface area with reference to the human equivalent dose (1000 mg/day)

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Hence they stimulate the accumulation of lipid intermediate metabolites [lysophosphatidic acid (LPA), diacylglycerol (DAG), acylcarnitines and ceramides], which promote defects of the insulin signaling pathway, exacerbate IR and stimulate several signaling pathways related to inflammation and oxidative stress, creating a second vicious-cycle of a feed-forward effect [17, 2123]

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