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ALOX15 dicer siRNA was purchased from IDT
Key nutrients for gut lining repair include L-glutamine (the primary fuel for enterocytes), zinc carnosine, collagen peptides, and short-chain fatty acids produced by beneficial gut bacteria
The RNAprotein complexes were captured on Dynabead protein A (Thermo Fisher, 10006D) at 4 C for 90 min, washed with NT2 buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM MgCl 2 and 0.05% NP-40) and then incubated in NT2 buffer containing DNase I (Qiagen, 79254) at 37 C for 10 min
A.GarmaevaT.VitvitskyV
Test Results: 4-6 business days once the lab receives the specimen
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