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Neutrophils were stained on fixed sections with monoclonal anti-Ly6G, whereas frozen samples were embedded in optimal cutting temperature compound, and sections were stained with monoclonal anti-CD301b or anti-TGM2 (See Table 2 for the antibodies used in this study)
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The injection protocols being shared on social media are largely based on anecdote and extrapolation rather than controlled trials examining skin outcomes
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