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Though it has a more distinct immunopathogenesis, PCD is known to have overlapping clinical features with both ACD and ICD, which can complicate diagnosis and management [34]

The protocols for these measurements were followed as described in the literature ( 2.12 Transcription of target genes (IL-2 and IFN- ) using qRT-PCR This step involved the preparation of liver tissues from experimental groups which were collected, placed in the Eppendorf tubes, immediately kept in liquid nitrogen, and stored at 80C till RNA extraction for determination of the gene expression of interleukin-2 (IL-2) and interferon-gamma (IFN-)

If you put it back in the fridge and take it out again, the counter does not reset

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