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This validation design allowed the construction of calibration curves (using the unspiked sample, SA2, and SA3) to accurately quantify the SA1 samples and to verify linearity via the coefficient of determination ( R 2 )

While it ensures direct systemic absorption, it is less patient-friendly and may result in reduced treatment adherence

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Moreover, the singlets corresponding to methyl groups of the C2 thiazolidine shifted from 1.41 and 1.35 ppm in AX to 1.52 and 1.16 ppm in DKP