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Then, TMB chromogen solution (CWBIO) was added, and the absorbance at 450 nm was measured via a SpectraMax plate reader (Molecular Devices)
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This can lead to contamination in medical settings and so using bacteriostatic water as part of the process helps to ensure that no biofilm forms on the peptides being reconstituted
These enzymes include malic enzyme 1 (ME1) and IDH1, which generate NADPH, and thus the levels and subcellular compartmentalization of malate and isocitrate dictate the activity of these enzymes
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