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L., Banerji M

If stopped suddenly, withdrawal effects may include mood changes, irritability, sleep problems, or physical symptoms (sometimes called discontinuation syndrome)

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And reacting at room temperature for 3h, adding 10mL of glacial methyl tert-ether, precipitating a white solid, centrifuging, adding methyl tert-ether to the product, washing for 3 times, washing for 2 times with 10mL of ethyl acetate, drying, and purifying by reversed-phase HPLC to obtain 85mg of semaglutide with the purity of 98.43%, wherein the liquid-phase chromatogram is shown in FIG