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Through the interaction of TNF and its receptors, key signaling pathways can be activated, such as the NF-B pathway, RANKL signaling, the extracellular signal-regulated kinase (ERK) signaling pathway, the tumor progression locus 2 (TPL2) pathway, and proapoptotic signaling

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The fully active state from 3DVA showed clear density for the basic loop of Lys471, Arg472 and Lys474 and an extended helix, indicating stronger membrane engagement, whereas the basic loop and the helix were not observed in the intermediate state (Fig