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MS spectra were acquired between 3501,250 m/z (molecular weight/valency) in high-resolution mode (40,000 resolution for full scans and 20,000 resolution for MS/MS scans) with a rolling collision energy using a 250 ms accumulation time/spectrum and 50 mDa mass tolerance, and the 10 most intense precursors per cycle were selected for fragmentation with a dynamic exclusion time of 30 s

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