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Characterization of the human intervertebral disc cartilage endplate at the molecular, cell, and tissue levels

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Urine samples collected for the measurement of F 2 -IsoPs were stored at 80C prior to shipment to Vanderbilt Eicosanoid Core Laboratory (Nashville, Tennessee, USA) for quantification of F 2 -IsoPs F 2 (PGF 2 ), free 8-iso-prostaglandin F 2 (8-iso-PGF 2 ), 2,3-dinor-5,6-dihydro-15-F 2t -IsoP and 5-series F 2 -IsoP by GC-negative ion chemical ionisation-MS employing stable isotope dilution as described in Milne et al

To minimize environmental exposure, containers should remain tightly sealed when not in use and handled according to established laboratory protocols