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Correct integration of linear DNA fragments was verified by colony PCR using primers 5-CCTGTGTAACTGATTAATCCTGC-3 (ADH2_up) and 5-GTCGACCTGCAGCGTACG-3 (S1/S3_reverse) for all N-terminal integrations and primers 5-GGAAGAATTGTTTACCTCGCTC-3 (ADH2 -fw) and S1/S3_reverse for C-terminal tagging