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On the other hand, some studies have shown that acetylcarnitine concentrations are significantly increased in patients with heart failure and in non-surviving patients after sepsis (Table 1) [38, 157]

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It's this whole shell game, but it sets precedent like we covered before

After centrifugation (1600 g, 4 C for 5 min), supernatant was diluted by two volumes of NET-2 buffer (50 mM Tris-HCl (pH=7.5), 150 mM NaCl, 0.05% NP-40, 1x protease and 1x phosphatase inhibitors) and pre-cleared by protein G Dynabeads at 4 C for 1 h

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