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Excess protein-linker compounds were removed by size-exclusion methods, yielding highly homogeneous conjugation products as determined by 12.5% SDS-PAGE and MALDI-TOF mass spectrometry

Documented allergies or contraindications to metformin (such as an eGFR below 30 mL/min/1.73m), a history of lactic acidosis, or severe adverse reactions to a prior GLP-1 RA may qualify a patient for an exception

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The severity of depression (measured by HAMD), levels of anxiety (assessed by HAMA), cognitive capabilities (evaluated through WCST), and serum S100B concentrations were thoroughly examined