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It should be noted, however, that the Grx activity of the enzyme was sustained without the C-terminal motif, as shown here and previously [11]

Gould N, Doulias P-T, Tenopoulou M, Raju K, Ischiropoulos H

SIRT3 is a member of the sirtuin family of histone deacetylases, which are primary mitochondrial protein deacetylases

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The wrong temperature for the wrong duration does not just reduce effectiveness