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Adding protease inhibitors such as 1mM PMSF to cell lysates does not inhibit binding or elution of polyhistidine-tagged proteins with the HisLink Resin and is highly recommended to prevent degradation of the protein of interest by endogenous proteases

Using transmission electron microscopy and other methods, we observed typical characteristics of ferroptosis, such as changes in mitochondrial morphology and Fe2 + accumulation

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1990 Oct;259(4 Pt 1):G524-9

Magnetic resonance imaging scans took place in a Discovery MR750 3T scanner in the Brain Health Imaging Centre at CAMH for approximately 1.5 h