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Tissue was rinsed with ice-cold phosphate-based saline (PBS) to remove feces and then digested in Dulbeccos Modified Eagle Medium (DMEM) containing 10% FCS, 10 mg/100 ml collagenase A, 2 mg/100 ml Dispase II (both Roche/Sigma-Aldrich, Taufkirchen, Germany) and 7.7 mg/100 ml 1,4-Dithiothreit (Carl-Roth, Karlsruhe, Germany) for 10 min at 37 C

It can be combined with injectable GHK-Cu for enhanced results

Data availability Publicly available data sets (GSE114691 58 , GSE25906 59 , and GSE10588 60 ) were analyzed in this study and the data are available via geo/

Vitamin B12 Deficiency is more common than you might think, and its effects can range from mild fatigue to serious neurological complications

After fixation, the tissue samples were post-fixed in 1% osmium tetroxide solution (pH 7.4) and processed into epoxy resin