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Thus, folate builds up in the form of 5mTHF (the "methyl trap") and there is less folate in the forms CH 2 -THF and 10f-THF that are the substrates for thymidylate and purine synthesis, respectively
J Neurosci Methods 113:113 Zhang SY, Ong CN, Shen HM (2004) Critical roles of intracellular thiols and calcium in parthenolide-induced apoptosis in human colorectal cancer cells
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Protein lysates were immediately processed and stored at 80 C until used
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